Download Colorectal Cancer: Methods and Protocols by Steven M. Powell PDF

By Steven M. Powell

ISBN-10: 0896037673

ISBN-13: 9780896037670

ISBN-10: 1592590845

ISBN-13: 9781592590841

Instructs investigators in key genetic, mobile, and molecular organic tools of reading colorectal tumors. innovations and assays are defined intimately to permit researchers to test on colon tumors. so much chapters are written by means of scientists who pioneered the tools provided. assurance contains gross microdissection of colon tumors, institution of mobilephone strains and xenografted tumors, chromosomal analyses akin to FISH assays, mutational analyses of colon tumors and blood samples, gene expression analyses, and microsatellite instability checking out. encompasses a few colour photos. For researchers at a number of degrees, from graduate scholars to pros. Powell is affiliated with the college of Virginia overall healthiness process.

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DAY 2: Washing Solutions 1. 2X SSC: mix 100 mL 20X SSC with 900 mL distilled water. Keep at RT. 2. 50% Formamide/2X SSC: mix 20 mL formamide/16 mL distilled water/4 mL 20X SSC. Prepare 3 (40 mL) coplin jars. Heat to 45°C in a water bath. Discard after use. 3. 1X SSC: mix 5 mL 20X SSC with 995 mL distilled water. Keep at RT. 4. 1% Tween-20: 200 mL 20X SSC/1 mL Tween-20/dH2O to 1 L volume. Keep at RT. 3. 1. Preparation of Tumor Tissue Specimens for FISH 1. Make touch preps using fresh tissue sample, frozen tissue sample or frozen tissue section.

Toward this goal, various investigators have used physical microdissection techniques to isolate specific cells (for example refs. 3–5, and many others). An alternative to physical isolation are in situ hybridization techniques. Unfortunately, the sensitivity of in situ techniques are limited and not generally useful for mutation analysis of single-copy genes. In situ amplification techniques have been useful for the analysis of viral infections (6,7). From: Methods in Molecular Medicine, vol.

1997) Assignment of trophoblast/endometrial epithelium cell adhesion molecule trophinin gene Tro to human chromosome bands Xp11. 21-11. 22 by in situ hybridization Cytogenet. Cell Genet. 79(1–2), 123–124. 2. Pack, S. , Ledbetter, D. , and Fukuda, M. N. (1999) Assignment of bystin gene BYSL to human chromosome band 6p21. 1 by in situ hybridization Cytogenet. Cell Genet. 83, 76–77 3. , Raap, A. , Tanke, H. , and Geraedts, J. P. M. (1989) Detection of chromosome aberrations in interphase tumor nuclei by non-radioactive in situ hybridization.

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Colorectal Cancer: Methods and Protocols by Steven M. Powell

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