By Ashok Pandey, Sangeeta Negi, Carlos Ricardo Soccol
ISBN-10: 0444636625
ISBN-13: 9780444636621
ISBN-10: 0444636730
ISBN-13: 9780444636737
Current advancements in Biotechnology and Bioengineering: creation, Isolation and Purification of commercial Products offers large insurance of latest advancements, cutting-edge applied sciences, and capability destiny developments, targeting commercial biotechnology and bioengineering practices for the creation of commercial items, comparable to enzymes, natural acids, biopolymers, and biosurfactants, and the techniques for separating and purifying them from a construction medium.
During the previous couple of years, the instruments of molecular biology and genetic and metabolic engineering have rendered super advancements within the creation of commercial items through fermentation. established by way of business product classifications, this booklet offers an outline of the present perform, prestige, and destiny capability for the construction of those brokers, besides stories of the economic state of affairs in relation to their production.
- Provides details on commercial bioprocesses for the creation of microbial items by way of fermentation
- Includes separation and purification procedures of fermentation products
- Presents financial and feasibility exams of a few of the strategies and their scaling up
- Links biotechnology and bioengineering for business method development
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Extra info for Current Developments in Biotechnology and Bioengineering. Production, Isolation and Purification of Industrial Products
Example text
0. 0 with a half-life of 13 h, 8 h, and 3 h 40 min at 60, 65, and 70 C, respectively. 3 Yeast Sources Candida famata [31], Pichia subpelliculosa [32], and Saccharomyces diastaticus [33] are some of the yeast used for production of GA. GA production has also been reported from yeast strains of Saccharomycopsis fibuligera [34] and Lipomyces starkeyi HN-606 [35]. Mohamed et al. isolated C. famata from traditional Moroccan sourdough for production of GA [31]. Starch enhanced GA production, with maximum GA activity at 5 g/L.
2 mg/mL min, respectively [94]. Nguyen et al. reported Km and Vmax of a-amylase from T. 19 U/mg, respectively [47]. For industrial use, the stability of an enzyme in the presence of metal salts is very important. In fact more than 75% of enzymes require metal ions to express their full catalytic activities. The optimum concentration of metal ions can be used to enhance the catalytic activity of an enzyme in that metal ions act as cofactors of many enzymes, but a high concentration of metal ions generally causes denaturation of the enzyme [95].
The enzymes degrade starch into smaller dextrins, which are subsequently fermented by the yeasts. This generates additional sugar in the dough, which improves the crust color, taste, and toasting properties of bread. a-Amylase acts as an antistaling agent and improves the softness retention and shelf life of baked foods. Generally, a-amylases from B. stearothermophilus are used commercially in the baking industry [97]. 7 Alcohol Production For the production of ethanol from starch, it has to be solubilized and then submitted to two enzymatic steps to obtain fermentable sugars.
Current Developments in Biotechnology and Bioengineering. Production, Isolation and Purification of Industrial Products by Ashok Pandey, Sangeeta Negi, Carlos Ricardo Soccol
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