By T. Brzozowski
ISBN-10: 9535105213
ISBN-13: 9789535105213
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Extra resources for New Advances in the Basic and Clinical Gastroenterology
Sample text
Prebiotics are found naturally in a number of materials. , 2005). , 2001; Ziemer & Gibson, 1998). , 2002). Raffinose and stachyose can be found in soyabeans and other leguminous seeds and pulses (Voragen, 1998). , 2001; Newburg, 2000; Petschow & Talbott, 1991). Human milk contains more galactooligosaccharides than cow’s milk. , 2004). Taking foods containing prebiotic oligosaccharides is not enough for modulation of gut flora as they are present in only small concentrations in these foods. , 1999).
G extreme temperatures, high pressure, shear forces) they encounter during their production at the industry level or in the GIT (gastric 30 New Advances in the Basic and Clinical Gastroenterology acids and bile salts) (Siuta-Cruce & Goulet, 2001). , 2002). Oxygen toxicity is another major problem in the survival of probiotic bacteria in dairy foods. , 2001). Manufacturers of probiotics are facing the challenge that they should produce probiotic cultures that can survive for long periods, and are resistant to acidity in the upper intestinal tract so that they can reach the colon in high numbers to colonize the epithelium.
6 Methods for quantification of probiotic cultures The methods used for detection of viable probiotic cells include conventional plate counts (culture dependent) and molecular techniques (culture-independent). , 2000). Isolation media used may be insufficiently selective, affecting the reproducibility of results (Roy, 2001). , 2003). , 2006). , 2001) of each cell individually (Bunthoff & Abee, 2002). Microplate scale fluorochrome staining assay is appropriate for assessing viability of fresh, freeze-dried and stressed cells.
New Advances in the Basic and Clinical Gastroenterology by T. Brzozowski
by Donald
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