By Byron Blagburn
Diagnostic guide that includes inner parasites of canines and cats.
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Add a coverslip. 3. Examine the entire slide. Thickness of the smear should allow reading newsprint placed beneath it. Note: Iodine can be added to the direct smear at the coverslip margin to stain motile protozoa, cysts or larvae—or for flotation techniques, the coverslip can be added to a drop of iodine already placed on the slide. 62 Sedimentation Sample preparation for sedimentation Simple straining procedures can separate large debris from parasites and from smaller debris. 1. Mix feces thoroughly with water in a clean disposable cup.
Muscles, eyes and central nervous system) 46 DIPYLIDIUM CANINUM Adult worms in small intestine Prepatent period: 2-3 weeks Patent period: several months *On occasion: dog biting lice Trichodectes canis Infected adult flea ingested by dog 47 Infectious larvae develops as larval flea develops into adult Posterior (gravid) (gravid) segments segments individual egg or individual egg packets packets are are passed passedininfaeces feces Segmentsand andegg eggpackets packetsinin Segments feces and area faeces and on on fur fur in perineal area Eggs ingested by larval flea* INTERNAL PARASITES OF DOGS AND CATS PARASITE LIFE CYCLES INTERNAL PARASITES OF DOGS AND CATS DIROFILARIA IMMITIS Larvae mature into adults in the pulmonary arteries and the right heart; female adults release microfilariae in the blood Prepatent period: 6 months Patent period: several years Effects of immature heartworms: Disease associated with Dirofilaria immitis may not be limited to mature adult worms.
Notes: Centrifuging sedimentation samples can increase test speed and improve performance. Some larvae can be recovered in a flotation procedure. A sedimentation preparation can be difficult to read due to large amounts of debris on the slide. 63 Sedimentation procedure: 1. Centrifuge or let preparation stand until sediment forms. 2. Remove most of the liquid above the sediment. 3. Place a drop of the sediment on a slide, then add a coverslip and examine. 20, measured with a hydrometer. 20. • Remember that simple flotation may underestimate or misdiagnose low parasite burdens.
Internal Parasites of Dogs & Cats: Diagnostic Manual by Byron Blagburn
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